The 51-year-old royal wore a red power suit in an appearance at the Museo Nacional Centro de Arte Reina Sofía with her husband King Felipe, 55.
The mother of two’s stylish two-piece suit featured subtle shoulder-pads and light tortoiseshell buttons on the sleeves and jacket.
Letizia paired her red jacket with an oyster-coloured silk blouse with slight ruching around the neckline, and completed her chic ensemble with pink block high heels.
Taking a leaf out of the Princess of Wales’s book, Letizia styled her glossy brown hair in loose waves for the occasion.
The Spanish royal kept her jewellery minimal, opting for an elegant pair of diamond dangly earrings.
What’s more, the Spanish queen donned her signature bronze smokey eye look and finished off her makeup with a nude lipstick.
Meanwhile, King Felipe was dapper in a grey pinstripe suit and red tie, which perfectly completed his wife’s outfit.
The exhibition officially opens tomorrow at the Museo Nacional Centro de Arte Reina Sofía and runs until March 2024.
The national museum has had ties to the Royal Family since it was opened in 1992 and was named after King Felipe’s mother Sofia.
The queen comes from humble beginnings. Born on September 15, 1972, in Oviedo, northern Spain, Letizia grew up in a middle-class family and attended a public school.
While her mother, Maria Rodriguez, was a nurse, Letizia took after her father, Jesús José Ortiz Álvarez, and stepmother Ana Togores, who are both journalists, and studied communications at university in Madrid.
She then got her Masters in broadcast journalism and after a stint at Asturian daily paper, La Nueva España, she went on to work for ABC, a popular national newspaper that enjoys the third largest circulation in Spain.
Next came a stint at Spanish news agency EFE before a move to Guadalajara in Mexico saw her take on a role on local paper, Siglo 21.
Back in Spain a year later, she worked for the Spanish version of Bloomberg, a news channel and agency specialising in economics, before moving to CNN+.
Before she met Felipe of Spain, Letizia was married to high school literature teacher Alonso Guerrero Perez, who was nine years her senior.
The couple wed in 1998 following a ten-year romance, with the pair meeting when the future Spanish queen was just 16.
They tied the knot in a civil ceremony in the town of Almendralejo, Badajoz, but divorced the following year.
By the time she met her husband-to-be, Felipe, in 2002, Letizia was working for popular TV channel 24 Horas, where along with anchoring the popular Telediario 2 evening news bulletin she reported on a wide range of breaking news event.
As a news anchor, she reported live from Ground Zero after the 9/11 attacks in 2001.
She met Prince Felipe the following year – at the site of an oil spillage in Northern Spain. The year after first meeting Felipe, Letizia quit her job and a few days later their engagement was announced.
Letizia and Felipe’s marriage in Madrid in 2004 prompted an upswing in support for the royal family, and her habit of championing High Street brands like Zara proved popular. She and Felipe had two daughters, Leonor in 2005 and Sofia in 2007.