Bloomberg reported on Friday night that Meadows, 61, was confirmed to be infected with the virus, along with at least four other White House aides.
As with previous outbreaks, the White House tried to keep it secret, CNN and Bloomberg reported.
On Friday 125,000 Americans tested positive – a new record, and the third consecutive day with more than 100,000 new cases every day.
It was unclear when he tested positive.
Mark Meadows, 61, has tested positive for coronavirus, it emerged on Friday
Meadows, right, has been one of the strongest supporters of Trump’s anti-mask policy
His boss tested positive on October 1 and is thought unlikely to become infected again.
Meadows was with the president throughout his treatment at the Walter Reed medical center.
The White House has been battling a coronavirus outbreak since September 26, when a Rose Garden ‘super spreader event’ was held – the nomination of Amy Coney Barrett.
Meadows was with Trump on his final two-day, seven state, 10 rally campaign swing where he was spotted at the events without wearing a face mask.
On that trip, Meadows had close contact with members of the Trump family who have not been diagnosed with COVID – including Jared Kushner and Ivanka Trump.
Additionally, Meadows was at the White House on election night where he attended a party in the East Wing filled with prominent Trump supporters, including the president’s personal lawyer Rudy Giuliani, 76.
Organizers said that everyone would be tested for COVID-19 on entry.
Earlier on Tuesday Meadows joined Trump at the campaign headquarters in Arlington, Virginia, and was again not wearing a mask.
On Thursday he was in the White House.
Meadows, circled, was with Trump on Tuesday morning at campaign HQ
Meadows has been one of the most adamant anti-maskers in the Trump administration, and insiders say he is rarely seen wearing a face mask, in deference to Trump.
On October 12, outside Coney Barrett’s Senate hearing, Meadows was asked by reporters to keep his face mask on while addressing them.
‘I’m not going to talk through a mask,’ he said, and walked off.
In May, Meadows flouted CDC restrictions by hosting his daughter’s 70-person indoor wedding in Atlanta, despite major coronavirus lockdowns and local ordinances blocking large gatherings.
Meadows is pictured accompanying Trump to Walter Reed on October 2